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Le Norcy, Tiffany; Niemann, Hendrik; Proksch, Peter; Linossier, Isabelle; Vallee-rehel, Karine; Hellio, Claire; Fay, Fabienne. |
Dibromohemibastadin-1 (DBHB) is an already known potent inhibitor of blue mussel phenoloxidase (which is a key enzyme involved in bioadhesion). Within this study, the potentiality of DBHB against microfouling has been investigated. The activity of DBHB was evaluated on key strains of bacteria and microalgae involved in marine biofilm formation and bioassays assessing impact on growth, adhesion and biofilm formation were used. To assess the efficiency of DBHB when included in a matrix, DBHB varnish was prepared and the anti-microfouling activity of coatings was assessed. Both in vitro and in situ immersions were carried out. Confocal Laser Scanning Microscopy (CLSM) was principally used to determine the biovolume and average thickness of biofilms developed... |
Tipo: Text |
Palavras-chave: Antifouling; Biodegradable coating; Hemibastadin; Sponges; Marine bacteria; Microalgae. |
Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00616/72812/72626.pdf |
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Carteau, David; Vallee-rehel, Karine; Linossier, Isabelle; Quiniou, Francoise; Davy, Romain; Compere, Chantal; Delbury, Maxime; Fay, Fabienne. |
The development of new antifouling coatings with respect to the marine environment is actually crucial. The aim of the present work is to concept an erodible paint formulated with biodegradable polyester as binders and which combines two modes of prevention: chemical and physical repelling of biofouling. This system is principally dedicated to disturb durable settlement of microfouling. Each component was chosen according to its specific properties: chlorhexidine is a bisdiguanide antiseptic with antibacterial activity, zinc peroxide is an inorganic precursor of high instable entities which react with seawater to create hydrogen peroxide, Tween 85 is a non ionic surfactant disturbing interactions between colonizing organisms and surface. Obtained results... |
Tipo: Text |
Palavras-chave: Environmental coatings; Antifouling; Active substances; Microfouling. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00168/27923/27273.pdf |
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Le Norcy, Tiffany; Niemann, Hendrik; Proksch, Peter; Tait, Karen; Linossier, Isabelle; Rehel, Karine; Hellio, Claire; Fay, Fabienne. |
Since the banning of several families of compounds in antifouling (AF) coatings, the search for environmentally friendly AF compounds has intensified. Natural sources of AF compounds have been identified in marine organisms and can be used to create analogues in laboratory. In a previous study, we identified that dibromohemibastadin-1 (DBHB) is a promising AF molecule, leading to the inhibition of the activity of phenoloxidase, an enzyme involved in the attachment of mussels to surfaces. This paper describes the activity of the DBHB on biofilm formation and its detachment and on bacterial adhesion and communication: quorum sensing. DBHB has an anti-biofilm activity without affecting adhesion of marine and terrestrial bacteria at a dose of 10 mu M.... |
Tipo: Text |
Palavras-chave: Biofouling; Biofilm; Bastadin derivative; Microfouling; Quorum sensing; Sponge. |
Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00616/72813/72581.pdf |
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